Journal: Cell

Article Title: A Genetically Defined Compartmentalized Striatal Direct Pathway for Negative Reinforcement

doi: 10.1016/j.cell.2020.08.032

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MOR Immunostar 24216 Rabbit polyclonal anti-tyrosine hydroxylase Millipore AB152 Chicken polyclonal anti-GFP Aves Labs GFP1020 Rabbit polyclonal anti-RFP Rockland 600-401-379 Rabbit monoclonal anti-HA-Tag Cell Signaling 3724S Mouse monoclonal anti-Parvalbumin Millipore MAB1572 Rabbit polyclonal anti-Somatostatin-14 Peninsula Laboratories T-4103 Goat polyclonal anti-ChAT Millipore AB144P Bacterial and Virus Strains AAV8-Ef1a-fDIO-GCaMP6m Laboratory of Karl Deisseroth N/A AAVdj-hSyn-Cre OFF /Flp ON -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55648 AAVdj-hSyn-Cre ON /Flp OFF -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55646 AAV8-EF1a-fDIO-Cre-p2A-mCherry Laboratory of Karl Deisseroth N/A AAV5-Ef1a-DIO-hChR2(H134R)-eYFP UNC N/A AAVdj-EF1a-fDIO-eYFP UNC N/A AAV8-hSyn-DIO-KORD-IRES-Mcitrine UNC N/A AAV2/8-Ef1a-fDIO-TVA-mCherry Laboratory of Z. Josh Huang N/A retroAAV2-CBA-fDIO-Cre Vigene Biosciences N/A AAV9-CAGGS-Flex-mKate-T2A-TVA HHMI Janelia Research Campus N/A AAV9-CAGGS-Flex-mKate-T2A-N2c-G HHMI Janelia Research Campus N/A Rbv-CVS-N2c-dG-GFP HHMI Janelia Research Campus Addgene 73461 AAV8-hSyn-DIO-mCherry Addgene Addgene 50459 Experimental Models: Organisms/Strains Mouse: Tg(Drd1a-cre)FK150Gsat/Mmucd (the “D1-Cre” line) MMRRC RRID: MMRRC_029178-UCD Mouse: B6.FVB(Cg)-Tg(Adora2a-cre) KG139Gsat/Mmucd (the “A2A-Cre” line) MMRRC RRID: MMRRC_036158-UCD Mouse: Tshz1-2A-FlpO This study N/A Mouse: Frt-Stop-Frt-TdTomato He et al., 2016 N/A Mouse: Pdyn-IRES-Cre (B6.Cg-129S-Pdyn tm1.1(cre)Mjkr/LowlJ) The Jackson Laboratory JAX: 027958 Mouse: Ai14 (B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J) The Jackson Laboratory JAX: 007908 Software and Algorithms ImageJ (Fiji) software NIH https://fiji.sc/ MATLAB Mathworks https://www.mathworks.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ Open in a separate window KEY RESOURCES TABLE Tshz1 labels a population of striatal direct pathway medium spiny neurons (dMSNs) Tshz1 -expressing ( Tshz1 + ) dMSNs are localized in the striosome Tshz1 + striosomal dMSNs represent punishment and drive negative reinforcement Pdyn labels another population of striosomal dMSNs mediating positive reinforcement

Techniques: Software

Journal: Cell reports

Article Title: Inactivation of medial entorhinal cortex selectively disrupts learning of interval timing

doi: 10.1016/j.celrep.2020.108163

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV8.hSyn.Jaws-KGC.GFP-ER2.WPRE.hGH Addgene 65014-AAV8 pAAV8-hSyn-EGFP Addgene 50465-AAV8 Chemicals, Peptides, and Recombinant Proteins NeuroTrace 530/615 Red Fluorescent Nissl Stain Invitrogen {"type":"entrez-nucleotide","attrs":{"text":"N21482","term_id":"1126652","term_text":"N21482"}} N21482 NeuroTrace 435/455 Blue Fluorescent Nissl Stain Invitrogen {"type":"entrez-nucleotide","attrs":{"text":"N21479","term_id":"1126649","term_text":"N21479"}} N21479 Experimental Models: Organisms/Strains Mouse: C57-BL6 Charles River N/A Software and Algorithms MATALB Mathworks https://www.mathworks.com/products/matlab.html ; P-Clamp Axon Instruments www.moleculardevices.com Open in a separate window KEY RESOURCES TABLE Mice learn interval timing task and report duration through immobile waiting Optogenetic inactivation of MEC disrupts learning of interval timing behavior Learning deficit revealed by MEC inactivation specifically during timing behavior MEC inactivation did not significantly impair timing behavior after learning

Techniques: Recombinant, Staining, Software

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: (A) Reginal (7p22 locus) association of GWAS variants with plasma cholesterol levels shows that common SNP rs1997243 (purple diamond) and GPR146 p.Gly11Glu (red circle) are significantly associated with plasma TC levels in humans.

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques: Clinical Proteomics

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: (A-D) Changes of plasma TG (A and C) and TC (B and D) after Poloxamer-407 injection in Gpr146 whole body knockout mice (Gpr146+/+ vs Gpr146−/−, n=12–14 mice per group, by Student’s t-test) and liver-specific knockout mice (Alb-Cre- vs Alb-Cre+, n=8 mice per group, by Student’s t-test) fed chow.

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques: Clinical Proteomics, Injection, Knock-Out

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: (A and C) Plasma TC levels of Gpr146 whole-body knockout male (A) and female (C) mice lacking LDLR fed chow or western diet (WD) for 16 weeks (n=6–10 mice per group, by one-way ANOVA).

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques: Clinical Proteomics, Knock-Out, Western Blot

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: (A) Schedule of AAV-mediated knockdown of GPR146 in mice lacking LDLR.

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques: Knockdown

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: (A) GSEA enrichment plot of MAPK_Signaling gene set (KEGG pathway database) in liver of Gpr146+/+ mice and Gpr146−/− littermates (n=3 mice per group) upon 6-hour refeeding after a 16-hour fast.

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques:

Journal: Cell

Article Title: GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis

doi: 10.1016/j.cell.2019.10.034

Figure Lengend Snippet: Key Resource Table

Article Snippet: To knock down hepatic Gpr146 in vivo , AAV8-scramble-shRNA (Vector Biolabs) or AAV8- Gpr146 -shRNA (Vector Biolabs) were administered to eight-week-old Ldlr −/− mice via retro-orbital injection, with a dose of 6 × 10 11 gc per male mouse and 4 × 10 11 gc per female mouse.

Techniques: Virus, Plasmid Preparation, Recombinant, Clinical Proteomics, Quantitation Assay, Microarray, Sequencing, Software